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. 2020 Nov 25;13:12181–12193. doi: 10.2147/OTT.S262896

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© 2020 Sun et al.

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Biological functions of GITR in vitro. (A) Expression of GITR in MKN45 and AGS cell line compared with GES1 cell line by qRT-PCR. (B) Expression of GITR in MKN45 and AGS cell line compared with GES1 cell line by ELISA. (C and D) CCK8 assay for GITR knock down MKN45 and AGS cell line. (E and F) Caspase 3/7 apoptosis assay for GITR knock down MKN45 and AGS cell line. (G and H) CCK8 assay for GITR overexpression MKN45 and AGS cell line. (I and J) Caspase 3/7 apoptosis assay for GITR overexpression MKN45 and AGS cell line. (K) Transwell assay for GITR knock down MKN45 and AGS cell line. (L and M) Flow cell cytometry apoptosis for GITR knock down MKN45(L) and AGS(M) cell line. *indicates that P value<0.05.