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. 2020 Nov 25;13:12181–12193. doi: 10.2147/OTT.S262896

Figure 4.

Figure 4

Biological functions of SNHG6 and miR-325-3p in vitro. (A) Expression of SNHG6 in MKN45 cell line compared with GES1 cell line by qRT-PCR. (B) Expression of miR-325-3p in MKN45 cell line compared with GES1 cell line by qRT-PCR. (C) CCK8 assay for SNHG6 knock down MKN45 cell line. (D) Caspase 3/7 apoptosis assay for SNHG6 knock down MKN45 cell line. (E) Flow cell cytometry apoptosis for SNHG6 knock down MKN45 cell line. (F) CCK8 assay for miR-325-3p overexpression MKN45 cell line. (G) Caspase 3/7 apoptosis assay for miR-325-3p overexpression MKN45 cell line. (H) Flow cell cytometry apoptosis for miR-325-3p overexpression MKN45 cell line. (I) Rescue experiments for CCK-8 in MKN45. Group: NC, miR-325-3p knock down, SNHG knock down, miR-325-3p knock down + SNHG knock down. (J) Rescue experiments for caspase 3/7 apoptosis in MKN45. Group: NC, miR-325-3p knock down, SNHG knock down, miR-325-3p knock down + SNHG knock down. *indicates that P value<0.05. (K) Western blot to test the expression of E-cadherin and N-cadherin in NC and SNHG-knock down MNK45 and AGS cell line.