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. 2020 Nov 16;11:583740. doi: 10.3389/fimmu.2020.583740

Figure 2.

Figure 2

Pathological changes and kinetics of the immune responses in the olfactory organ of trout infected with F. columnare. (A) Histological examination by H & E staining of olfactory organ from uninfected fish and F. columnare-infected rainbow trout at 7, 28 and 75 dpi. Black arrows indicate the width of LP at the tip region (100 μm from the lamellar tip) of the olfactory lamella. (B) The width of LP at the tip region the olfactory lamella in control fish and F. columnare -infected rainbow trout at 7, 28, 75 dpi counted from A. (C) Histological examination by A & B staining of olfactory organ from uninfected fish and F. columnare-infected rainbow trout at 7, 28 and 75 dpi. Red arrowheads indicate goblet cells. (D) The number of goblet cells at the olfactory lamella in control fish and F. columnare -infected rainbow trout at 7, 28 and 75 dpi counted from C. (E) Heat map illustrates quantitative real-time PCR results of relative mRNA levels for selected immune markers in F. columnare-infected fish versus control fish measured at 1, 2, 4, 7, 14, 21, 28 and 75 dpi (n = 6 per group) in the olfactory organ of rainbow trout. Data are expressed as mean fold increase in expression. (F) The expression levels of IgM, IgD and IgT at 2, 7, 28 and 75 dpi with F. columnare in olfactory organ of rainbow trout. (n = 6 fish per group). LP: lamina propria. Scale bars, 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA with Bonferroni correction). Data are representative of three independent experiments (mean ± SEM).