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. 2020 Nov 16;11:583740. doi: 10.3389/fimmu.2020.583740

Figure 4.

Figure 4

Immunoglobulin responses in the olfactory organ and serum from infected and survivor trout. (A) Western blot analysis of IgT-, IgM-, and IgD- specific binding to F. columnare in nasal mucus (dilution 1:2) from infected and survivor fish. (B, C) IgT-, IgM-, and IgD- specific binding to F. columnare in dilutions of nasal mucus from infected (B) and survivor fish (C), evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 12 per group). (D) Western blot analysis of IgT-, IgM-, and IgD- specific binding to F. columnare in serum (dilution 1:10) from infected and survivor fish. (E, F) IgT-, IgM-, and IgD- specific binding to F. columnare in dilutions of serum from infected (E) and survivor (F) fish, evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 12 per group). *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired Student t test). Data are representative of three independent experiments (mean ± SEM).