Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 16;11:583740. doi: 10.3389/fimmu.2020.583740

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Dong, Yin, Meng, Xu, Liu, Wang and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Proliferation of IgT⁺ B cells in the olfactory organ from survivor fish. (A, B) Immunofluorescence analysis of 5-ethynyl-2’-deoxyuridine (EdU) incorporation by IgT⁺ or IgM⁺ B cells in the olfactory organ of control (A) and survivor fish (B) and enlarged images of the areas outlined. Paraffinic sections of olfactory organ were stained for EdU (magenta), trout IgT (green), trout IgM (red), and nuclei (blue) detection. White arrowheads point to cells double stained for EdU and IgT. NC, nasal cavity; OE, olfactory epithelium; LP, lamina propria. Scale bars, 20 μm. (C) Percentage of EdU⁺ cells from total olfactory organ cells in control and survivor fish counted from A and B (n = 6 fish per group). We have counted 18 fields or 19 fields in the control or survivor group, respectively. (D) Percentage of EdU⁺ cells from the total olfactory organ IgT⁺ and IgM⁺ B cell populations in control and survivor fish, statistically calculated from A and B. ***p < 0.001 (unpaired Student t test). Data in (C, D) are representative of at least three independent experiments (mean ± SEM).