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. 2020 Nov 16;11:583740. doi: 10.3389/fimmu.2020.583740

Figure 6.

Figure 6

Local IgT-, IgM-, and IgD-specific responses in olfactory organ explants of survivor fish. (A) The olfactory organ explants from control and survivor fish were cultured in medium (400 μl) for 7 days. Western blot analysis of IgT-, IgM-, and IgD-specific binding to F. columnare in the olfactory organ culture medium (dilution 1:2) from control and survivor fish. (B) IgT-, IgM-, and IgD-specific binding to F. columnare in dilutions of olfactory organ culture medium from control and survivor fish, evaluated by densitometric analysis of immunoblots and presented as relative values to those of control fish (n = 12 per group). *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired Student’s t-test). Data are representative of at least three independent experiments (mean ± SEM).