Figure 4.

Screening for Other Hits using HIV-1 Neutralization Assays

(A) Neutralization profiles of GO35 and GO52 against 14 HIV-1 and 2 control viruses. Assays were performed as duplicates (n = 2), and IC₅₀s were determined by fitting an asymmetric sigmoidal five-parameter dose-response curve (R² = 0.9757 for fit of GO52 against B41 virus).

(B) TZM-bl neutralization curves of GO35 and GO52 against B41 pseudotyped virus. Assays were performed as duplicates (n = 2), and error bars represent standard deviation.

(C) Neutralization activity of 80 small molecules against B41 HIV-1 and A-MLV viruses. All molecules were tested at a 30 μM final concentration. Assays were performed as duplicates (n = 2), and mean value is plotted. BMS-626529 and T20 (enfuvirtide) are known HIV-1 fusion inhibitors included as controls.

See also Figures S4 and S7.