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. 2020 Nov 16;11:590325. doi: 10.3389/fmicb.2020.590325

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Copyright © 2020 Gaeta, Bean, Miles, Carvalho, Alemán, Carvalho, Gregory and Ganda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heat map of the top 10 ECs selected by how well they distinguish (A) farm, (B) body site, and (C) farm given body site. Each box represents an individual sample and is shaded by its normalized read count value. Samples are stratified by farm (A,B) and body site from which the sample was taken [feces, rumen fluid, and deep nasal swab (DNS)]. ECs (Number: Class) = (A) 2.7.7.7: Nucleotidyltransferase, 2.7.7.8: Nucleotidyltransferase, 2.8.4.3: Methylsulfanyl transferase, 2.5.1.7: Alkyltransferase, 2.1.1.74: Methyltransferase, 2.4.2.14: Pentosyltransferase, 6.3.5.5: Carbon-nitrogen ligase, 1.1.1.49: Oxidoreductase, 3.1.3.16: Phosphoric - monoester hydrolase, 2.3.1.191: Acyltransferase. (B) 5.4.2.11: Phosphotransferase, 2.5.1.47: Alkyltransferase, 3.1.1.73: Carboxylic-ester hydrolase, 1.1.1.271: Oxidoreductase, 5.1.3.11: Epimerase, 1.1 1.1.22: Peroxidase, 1.1.1.192: Oxidoreductase, 4.2.1.45: Lyase, 3.2.1.80: Glycosylase, 1.5.1.43: Oxidoreductase. (C) 6.5.1.2: DNA ligase, 1.4.7.1: Oxidoreductase, 6.3.5.4: Carbon-Nitrogen Iigase, 4.2.2.23: Carbon-oxygen lyase, 2.7.1.162: Phosphotransferase, 3.4.21.1 16: Peptide hydrolase, 3.1.1.96: Carboxylic-ester hydrolase, 3.6.1.27: Nydr6lase, 3.2.1.156: Oiigosaccharide reducing-end xylanase, 1.5.1.43: Oxidoreductase.