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Measurement of cytotoxicity on THP-1-derieved-macrophages by secreted hIL-10. (A) E:T ratio optimization for THP-1-derived-macrophages versus PK(15) cells in cell to cell cytotoxicity assay. (B) Result of the cytotoxicity assay in IL-10 expressing PK(15) cells. (C) Cytotoxicity assay after treatment with rhIL-10-dose-dependent manners. Data are presented as mean ± S. D (n = 3 biological replicates). (*P < 0.05, **P < 0.005).
