Figure 1.

JQ1 inhibited the growth of oesophageal cancer cells. A, Box plots of BRD2, BRD3 and BRD4 expression in tumour tissue (Red, patient number = 182) vs normal tissue (grey, patient number = 286) acquired from GEPIA2 website. B, Four oesophageal cancer cell lines were treated with indicated JQ1 for 72 hours. The cell viability was assayed by CCK‐8. The data represented the mean of triplicate experiments. C, Expression levels of BRD2, BRD3 and BRD4 in oesophageal cancer cell lines using real‐time PCR. The mRNA expressions were normalized by the housekeeping gene GAPDH. The relative expression of y‐axis presents the difference of threshold cycles between target transcript and GAPDH. The results were represented as the average of three experiments. D, Protein levels of BRD4 in various oesophageal cancer cell lines using immunoblotting. E, KYSE450 cells were treated with different concentrations of JQ1 and observed under a microscope