Figure 4.

Knockdown of BRD4 induced senescence and down‐regulated AURKA and AURKB. KYSE450 cells were infected with lentivirus expressing BRD4 shRNA for 6 d. β‐Gal staining was assayed in (A) or total protein was extracted for immunoblotting in (B). C, KYSE450 cells were explored to JQ1 at different concentrations, and the transcriptional levels of potential targets were examined using qPCR. The data were presented as means ± SD. *P ≤ .05, ****P ≤ .001. D, BRD4 inhibition decreased AURKA and AURKB protein in a dose‐ and time‐dependent pattern. GAPDH served as a loading control. E, BRD4 knockdown suppressed the protein levels of AURKA and AURKB. F, Representative ChIP‐quantitative PCR assay of KYSE450 cells investigated BRD4 binding to AURKA and AURKB promoter with the presence or absence of JQ1. **P ≤ .01, ***P ≤ .005, ****P ≤ .001. G, The differential expressions of AURKA and AURKB in oesophageal cancer and normal tissues obtained from TCGA database. ** P ≤ .01