FIGURE 4.

circ_SEPT9 acts as sponges for miR‐1225. A, Level of miR‐1225 was detected after silence of circ_SEPT9 in SCC‐15 and HSC‐3 cells. B, Level of miR‐1225 was detected after overexpression of circ_SEPT9 in SCC‐9 and UM1 cells. C, Pull down of circ_SEPT9 by biotin‐labelled wide‐type or binding site–mutated miR‐1225 in SCC‐15 cell lysis. D, Pull down of circ_SEPT9 by biotin‐labelled wide‐type or binding site–mutated miR‐1225 in UM1 cell lysis. E, Negative correlation between circ_SEPT9 and miR‐1225 in OSCC specimens detected by qRT‐PCR. F, qRT‐PCR analysis for the expression of miR‐1225 in OSCC cancer tissue and the adjacent normal tissue