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. 2020 Oct 14;24(22):13266–13277. doi: 10.1111/jcmm.15943

FIGURE 6.

FIGURE 6

circ_SEPT9/miR‐1225 regulate the mRNA and protein level of PKN2. A, Diagram of predicted interactions between PKN2 mRNA and miR‐1225. B, qRT‐PCR and Western blot were used to detect the mRNA and protein level of PKN2 after silence of circ_SEPT9 in SCC‐15. C, qRT‐PCR and Western blot were used to detect the mRNA and protein level of PKN2 after overexpression of circ_SEPT9 in UM1. D, Luciferase activities of circ_SEPT9 in SCC‐15 and UM1 cells after indicated treatment. E, qRT‐PCR was used to detect the mRNA level of PKN2 in SCC‐15 and UM1 cells after indicated treatment. F, Western blot was used to detect the protein level of PKN2 in SCC‐15 and UM1 cells after indicated treatment. G, Positive correlation between circ_SEPT9 and PKN2 in OSCC specimens was shown (Right). Negative correlation between miR‐1225 and PKN2 in OSCC specimens was shown (Left). H, qRT‐PCR analysis for the expression of PKN2 in OSCC tissue and the adjacent normal tissue