FIGURE 8.

circ_SEPT9/miR‐1225/PKN2 axis were confirmed in vivo. A, qRT‐PCR was performed to detect the expression of circ_SEPT9 in tumours of nude mice. B, qRT‐PCR was performed to detect the expression of miR‐1225 in tumours of nude mice. C, qRT‐PCR was performed to detect the expression of PKN2 in tumours of nude mice. D, Western blot was performed to detect the expression of PKN2 in tumours of nude mice. E, Relative protein expression of PKN2 was calculated by ImageJ. β‐Actin was used as the loading control