Figure 6.

DSC inhibits NF‐κB activation. Colitis was induced as described in Materials and Methods and treated with or without DSC (50 mg/kg). A, Representative bands and quantitative analysis of p‐NF‐κB p65 in colonic tissues, NF‐κB p65 was used as loading control. B, Colitis was induced as described in Materials and Methods and treated with or without lentiviral Nox4 shRNA. Representative bands and quantitative analysis of p‐NF‐κB p65 in colonic tissues, NF‐κB p65 was used as loading control. BMDM was stimulated with or without DSC (50 μmol/L) as described in Materials and Methods. C, Representative bands and quantitative analysis of p‐NF‐κB p65 in BMDM and NF‐κB p65 was used as loading control. D, Quantitative analysis of NF‐κB p65 DNA binding activity in BMDM. Data shown are means ± SEM of n = 8 in each group. *P < 0.05 vs Control cell or mice (CTL), ^#P < 0.05 vs DSS‐treated mice or LPS‐stimulated cells