Skip to main content
. 2020 Oct 15;24(22):13208–13222. doi: 10.1111/jcmm.15933

Table 1.

Sequences used for qRT‐PCR, cell transfection and dual‐luciferase reporter assay

Gene Name Sence primer (5′ → 3′) Antisence primer (5′ → 3′)
miR‐203 CGCCGGTGAAATGTTTAGGA CAGCCACAAAAGAGCACAAT
miR‐203 stem‐loop CCTGTTGTCTCCAGCCACAAAAGAGCACAATATTTCAGGAGACAACAGGCTAGTGG
U6 CTCGCTTCGGCAGCACA AACGCTTCACGAATTTGCGT
URP TGGTGTCGTGGAGTCG
miR‐203 mimic GUGAAAUGUUUAGGACCACUAG AGUGGUCCUAAACAUUUCACUU
Negative control UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT
rZEB1‐siRNA CCAGCGGUCAUGAUGACAATT UUGUCAUCAUGACCGCUGGTT
GCUCACACAUAAGCAGUAATT UUACUGCUUAUGUGUGAGCTT
GCCAACAGUUGGUUUGGUATT UACCAAACCAACUGUUGGCTT
rSurvivin‐siRNA CCGAGAAUGAGCCUGAUUUTT AAAUCAGGCUCAUUCUCGGTT
GGAUCUACACCUUCAAGAATT UUCUUGAAGGUGUAGAUCCTT
GCGCCUUCCUUACAGUCAATT UUGACUGUAAGGAAGGCGCTT
rTGF‐β1‐siRNA CUGAGUGGCUGUCUUUUGATT UCAAAAGACAGCCACUCAGTT
AGACAUCACACACAGUAUATT UAUACUGUGUGUGAUGUCUTT
CAGCUGUACAUUGACUUUATT UAAAGUCAAUGUACAGCUGTT
rSurvivin TTGGCTCTTTGTTTGTCCAGTTTCA GGCTTCATCCACTGCCCTACC
rβ‐actin TGAGCTGCGTGTGGCCCCTGAG GGGGCATCGGAACCGCTCATTG
WT Survivin‐3′‐UTR CTGTTCCTGAGAAAATAAAAAGCCTGTCATTTCAAACACTGCTGTGGACCCTACTGGG
Mu Survivin‐3′‐UTR CTGTTCCTGAGAAAATAAAAAGCCTGTGAATACAAACACTGCTGTGGACCCTACTGGG

Abbreviations: Mu, mutant; r, rat; WT, wild‐type.