Table 1.
Gene Name | Sence primer (5′ → 3′) | Antisence primer (5′ → 3′) |
---|---|---|
miR‐203 | CGCCGGTGAAATGTTTAGGA | CAGCCACAAAAGAGCACAAT |
miR‐203 stem‐loop | CCTGTTGTCTCCAGCCACAAAAGAGCACAATATTTCAGGAGACAACAGGCTAGTGG | |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
URP | TGGTGTCGTGGAGTCG | |
miR‐203 mimic | GUGAAAUGUUUAGGACCACUAG | AGUGGUCCUAAACAUUUCACUU |
Negative control | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
rZEB1‐siRNA | CCAGCGGUCAUGAUGACAATT | UUGUCAUCAUGACCGCUGGTT |
GCUCACACAUAAGCAGUAATT | UUACUGCUUAUGUGUGAGCTT | |
GCCAACAGUUGGUUUGGUATT | UACCAAACCAACUGUUGGCTT | |
rSurvivin‐siRNA | CCGAGAAUGAGCCUGAUUUTT | AAAUCAGGCUCAUUCUCGGTT |
GGAUCUACACCUUCAAGAATT | UUCUUGAAGGUGUAGAUCCTT | |
GCGCCUUCCUUACAGUCAATT | UUGACUGUAAGGAAGGCGCTT | |
rTGF‐β1‐siRNA | CUGAGUGGCUGUCUUUUGATT | UCAAAAGACAGCCACUCAGTT |
AGACAUCACACACAGUAUATT | UAUACUGUGUGUGAUGUCUTT | |
CAGCUGUACAUUGACUUUATT | UAAAGUCAAUGUACAGCUGTT | |
rSurvivin | TTGGCTCTTTGTTTGTCCAGTTTCA | GGCTTCATCCACTGCCCTACC |
rβ‐actin | TGAGCTGCGTGTGGCCCCTGAG | GGGGCATCGGAACCGCTCATTG |
WT Survivin‐3′‐UTR | CTGTTCCTGAGAAAATAAAAAGCCTGTCATTTCAAACACTGCTGTGGACCCTACTGGG | |
Mu Survivin‐3′‐UTR | CTGTTCCTGAGAAAATAAAAAGCCTGTGAATACAAACACTGCTGTGGACCCTACTGGG |
Abbreviations: Mu, mutant; r, rat; WT, wild‐type.