FIGURE 4.

miR‐340‐5p inhibited glioma cell proliferation, migration, and invasion and promoted apoptosis by directly targeting ROCK1 mRNA. A, A schematic representation of putative miR‐340‐5p‐binding sites in ROCK1 mRNA. B, Dual‐luciferase reporter assays were performed to determine whether miR‐340‐5p directly binds ROCK1 mRNA. C, The expression levels of ROCK1 in glioma tissues and normal brain tissues were quantitated by qRT‐PCR. D, The expression levels of ROCK1 were negatively correlated with miR‐340‐5p expression levels in glioma tissue samples. E, F, The expression levels of ROCK1 were determined in U87 and U251 cells transfected with miR‐340‐5p or co‐transfected with miR‐340‐5p and a ROCK1‐overexpressing plasmid. G‐J, Glioma cell proliferation, apoptosis, migration and invasion were then measured by CCK‐8, flow cytometry and transwell assays, respectively. ^* P < .05