FIGURE 6.

eNOS and nNOS expression with the NO and cGMP concentration in the corpus cavernosum. (A) Representative results of Western blot analysis of eNOS and nNOS in the rats of all five groups. (B) Expression of eNOS in all five groups with β‐actin as the loading control is presented as a bar graph. (C) Expression of nNOS in all five groups with β‐actin as the loading control is presented as a bar graph. (D) The black rectangle indicates the area of the corpus cavernosum selected for comparison. (E) The eNOS level is defined as eNOS IOD/area according to the immunofluorescence results. (F) The nNOS level is defined as nNOS IOD/area according to the immunofluorescence results. (G) Representative results of immunofluorescence analysis of eNOS and nNOS in all five groups. Scale bars = 50 μm. (H) Representative results of immunohistochemistry analysis of eNOS and nNOS in all five groups. The arrows indicate eNOS and nNOS expression (Because the expression levels of eNOS and nNOS in DMED group were very low, the coloring was very light). Scale bars = 50 μm. (I) The NO production in all five groups was determined with an assay kit. (J) The cGMP concentration in all five groups was determined with an assay kit. Data are expressed as the mean ± standard deviation. ^& P < .05 compared with the Con group. ^# P < .05 compared with the DMED group. ^* P < .05 compared with the DMED + CCSMC‐EXO group. CCSMC‐EXOs: exosomes derived from corpus cavernosum smooth muscle cells; BMSC‐EXOs: exosomes derived from bone marrow stem cells; ADSC‐EXOs: exosomes derived from adipose‐derived stem cells; DMED: diabetes mellitus‐induced erectile dysfunction; DAPI: 4',6‐diamidino‐2‐phenylindole; eNOS: endothelial nitric oxide synthase; nNOS: neuronal nitric oxide synthase; NO: nitric oxide; cGMP: cyclic guanosine monophosphate; IOD: integral optical density; IOD/area: mean optical density