Figure 3.

Double deletion of ACE2 and Mas receptor enhances Ang II‐induced renal fibrosis. A, Real‐time PCR detects α‐smooth muscle actin (α‐SMA) and collagen I (Col. I) mRNAs. B, Western blot analysis of α‐SMA and Col. I. C, Col. I deposition detected by immunohistochemistry. Data represent the mean ± SEM for groups of 5‐12 mice. *P < .05, **P < .01, ***P < .001 vs. WT + SL; ^# P < .05, ^## P < .01, ^### P < .001 vs. WT + Ang II; ^$ P < .05, ^$$ P < .01, ^$$$ P < .001 vs. ACE2 KO + Ang II and Mas KO + Ang II. Scale bar, 100 μm