Figure 5.

Double deletion of ACE2 and Mas receptor enhances Ang II‐induced activation of AT1, ERK1/2, and Smad3 signalling in the hypertensive kidney. A, Western blot analysis of Ang II receptor type 1 (AT1), phospho‐ERK1/2 (p‐ERK1/2), phospho‐Smad3 (p‐Smad3). B, Immunohistochemistry of p‐Smad3 nuclear translocation. Data represent the mean ± SEM for groups of 6‐12 mice. *P < .05, ***P < .001 vs. WT + SL; ^# P < .05, ^## P < .01, ^### P < .001 vs. WT + Ang II; ^$ P < .05, ^$$ P < .01, ^$$$ P < .001 vs. ACE2 KO + Ang II and/or Mas KO + Ang II. Scale bar, 50 μm