Figure 7.

Double deletion of ACE2 and Mas receptor enhances Ang II‐induced activation of nuclear factor κB (NF‐κB) signalling in the hypertensive kidney. A, Western blot analysis of Smad ubiquitination regulatory factors 2 (Smurf2), Smad7, phospho‐IκBα (p‐IκBα), and phospho‐NF‐κB/p65 (p‐p65). B, Immunohistochemistry of NF‐κB/p‐p65 nuclear translocation. Data represent the mean ± SEM for groups of 6‐12 mice. *P < .05, ***P < .001 vs. WT + SL; ^# P < .05, ^## P < .01, ^### P < .001 vs. WT + Ang II; ^$ P < .05, ^$$$ P < .001 vs. ACE2 KO + Ang II and Mas KO + Ang II. Scale bar, 50 μm