FIGURE 2.
LSC frequency was increased in Tgif1−/− mice with AML compared to Tgif1 +/+ mice. Lin‐ bone marrow cells (Lin‐HSPCs) from Tgif1+/+ and Tgif1−/− mice were transduced with MLL‐AF9‐GFP retrovirus and transduced cells transplanted into sub‐lethally irradiated Tgif1+/+ and Tgif1−/− recipients, respectively. At a predetermined time, serial dilutions of spleen cells from mice with leukaemia then collected and transplanted into secondary recipients. Kaplan‐Meier analysis of survival is plotted as a function of number of transplanted spleen cells. A, 1 × 106; B, 1 × 105; C, 1 × 104; D, 1 × 103