FIGURE 4.

Mice with Tgif1^−/− HSPCs developed more aggressive disease and had inferior survival compared to those with Tgif1^+/+ HSPCs in BCR‐ABL‐induced CML. Enriched Lin^‐ c‐Kit⁺ cells from Tgif1^+/+, Tgif1^−/− or Tgif1^± bone marrow cells (HSPCs) were transduced with BCR‐ABL‐GFP retrovirus and transduced cells transplanted into lethally or sub‐lethally irradiated C57BL/6J recipients to induce CML. Six weeks after transplant, flow cytometry was used to analyse A, percentage of GFP⁺ cells in PB; B, percentage of GFP⁺ cells in bone marrow; C, number of GFP⁺ LSK cells in bone marrow. Recipient mice were D, lethally irradiated; E and F, sub‐lethally irradiated in fractional survival curves