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. Author manuscript; available in PMC: 2020 Nov 30.
Published in final edited form as: J Med Chem. 2019 Jul 3;62(14):6814–6823. doi: 10.1021/acs.jmedchem.9b00825

Figure 6.

Figure 6.

Binding of test compounds to BTN3A1-BFI as determined by fluorescence polarization assay. (A) Competitive inhibition curves HMBPP (1) in BTN3A1 domains: wt-BFI (IC50 = 19.9 μM), B30.2 (23.3 μM) and BFI(H381R) ( > 333 μM). Natural (B) and synthetic (C-D) butyrophilin ligands assayed in a competitive inhibition study. IC50 values reported in Table 1; n = 3 for all experiments. Data represents mean +/− SD.