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. Author manuscript; available in PMC: 2020 Nov 30.
Published in final edited form as: J Med Chem. 2019 Jul 3;62(14):6814–6823. doi: 10.1021/acs.jmedchem.9b00825

Figure 7.

Figure 7.

Analysis of butyrophilin ligands using mutants of the BTN3A1 diphosphate binding region. (A) HMBPP (1) displaces the GDP-BODIPY probe at different rates in mutants than in wt-BFI. (B) Curves of 1 displacement translated for range comparison. (C-D) Due to phosphonate probe interference, lower limit constraints for C-HMBP (5) and C-HMHP (10) were utilized. Lower limit seen using 1 were used as constraints for each individual mutant; raw files in Supplemental Information. Ranges and IC50 values reported in Table 2; n = 3 for all experiments.