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. 2020 Nov 24;11(6):e02641-20. doi: 10.1128/mBio.02641-20

TABLE 1.

Summary of metagenome-assembled genomes (MAGs) reconstructed from the metagenomes of the “Little Firn” glacier (LF) and nearby exposed soil (ES) that contain genes related to bacteriochlorophyll- or rhodopsin-based phototrophya

MAG Lineage
Size
(Mbp)
No. of
contigs
Completeness
(%)
Contamination
(%)
Phototrophy
(Sub)phylum Genus pufM PR ActR BR XR HeR
LF-bin-79 Alphaproteobacteria Rubritepida 3.78 585 95.51 3.73
LF-bin-280 Alphaproteobacteria Tardiphaga 5.55 642 91.96 7.88
LF-bin-283 Alphaproteobacteria Tardiphaga 4.50 785 92.36 8.02
LF-bin-46 Betaproteobacteria Ramlibacter 4.05 336 95.48 4.74
LF-bin-10 Gammaproteobacteria n.d. 2.55 141 92.16 2.56
LF-bin-172 Gammaproteobacteria n.d. 3.55 274 91.13 0.99
LF-bin-25 Actinobacteria Aeromicrobium 4.22 400 94.99 5.85
LF-bin-295 Actinobacteria Aeromicrobium 5.01 519 100 7.52
LF-bin-300 Actinobacteria n.d. 3.77 278 95.97 2.05
LF-bin-354 Actinobacteria n.d. 2.42 100 96.17 3.64
LF-bin-355 Bacteroidetes Ferruginibacter 3.68 394 91.9 1.07
LF-bin-258 Bacteroidetes Flavobacterium 2.95 172 94.92 5.24
LF-bin-351 Bacteroidetes Flavobacterium 3.28 269 94.16 3.86
LF-bin-500 Bacteroidetes n.d. 2.94 211 95.95 0.95
LF-bin-319 Bacteroidetes Pedobacter 3.00 296 90.03 3.99
LF-bin-240 Bacteroidetes Pseudopedobacter 3.34 191 91.39 6.54
LF-bin-127 Oligoflexia Bdellovibrio 2.86 186 91.39 0.9
LF-bin-439 Oligoflexia Bdellovibrio 3.27 192 95.21 4.68
LF-bin-57 Oligoflexia Bdellovibrio 2.76 42 90.32 0.9
LF-bin-124 Oligoflexia n.d. 2.33 259 90.69 4.95
LF-bin-178 Oligoflexia n.d. 4.04 82 91.89 1.79
LF-bin-339 Gemmatimonadetes n.d. 3.78 254 90.61 3.3
ES-bin-98 Alphaproteobacteria n.d. 3.50 252 95.22 2.55
ES-bin-147 Armatimonadetes n.d. 4.22 495 92.2 2.04
ES-bin-166 Bacteroidetes n.d. 4.04 68 94.46 0.71
ES-bin-26 Bacteroidetes n.d. 5.43 717 90.76 1.36
ES-bin-22 Cyanobacteria Leptolyngbya 5.12 603 91.39 3.97
ES-bin-313 Cyanobacteria n.d. 4.25 198 95.73 4.56
a

Only MAGs that are >90% complete with <10% contamination are shown. See Table S2 for the full list of MAGs (>50% complete and <10% contamination). PR, proteorhodopsin; XR, xanthorhodopsin; ActR, actinorhodopsin; BR, bacteriorhodopsin; HeR, heliorhodopsin (a recently discovered new type of rhodopsin [26]); n.d., not determined. The classification of rhodopsin genes was based on phylogenetic analysis using a comprehensive collection of rhodopsin genes as the reference data set (see Text S1).