FIG 1.

TsrA is a transcriptional repressor of a relatively small set of V. cholerae genes. (A to C) Volcano plot representations of differential expression analysis of the wild type versus the isogenic ΔtsrA mutant for V. cholerae O395-N1 (A), C6706 (B), and Haiti H1 (C) RNA-seq data sets. The x axis shows the log₂ fold change in expression, and the y axis shows the log odds of a gene being differentially expressed (ac, –log₁₀ P value). Genes are shown in blue if they pass the absolute confidence (ac) threshold of ≥1.3 (equivalent to a P value of ≤0.05, pink dotted line). The identities of the top three genes with the highest ac values, and the few genes with negative fold changes, are given. (D) Venn diagram of the number of genes that are members of the TsrA regulon in each of the strains.