Figure 2.

Average jitter percentage calculation and comparison among three recording systems. A, C Examples of recording from s-MW and i-MW respectively are presented in black trace. Zoomed-in window of a single ictal event which is indicated by blue dashed box is provided at the bottom for both examples. B, D Two ictal events that are specified by blue and red dashed boxes in A and C respectively are overlaid according to the alignment spike (red and blue trace). Two zoomed-in time windows at different time points of the recording are presented at the bottom of these two traces for both s-MW and i-MW (indicated by dashed boxes). Blue arrows indicate the spikes and red star specifies the alignment time point. Jitter between two selected events in B and D are 11% (low jitter) and 61% (high jitter), respectively. E, F Jitter evolutions within 1 h time window of recordings from i-MW and s-MW are illustrated in box plots. Values of jitter are binned into 5 min intervals. The whiskers represent the range of the data. The 25^th and 75^th percentiles of the samples are contained within the top and bottom of the boxes. The line in the middle represent the median. G Jitter at the first 15 min and the last 15–20 min of the recordings are averaged and compared through paired t-test both for s-MW and i-MW samples. The initial and last 15–20 min then compared between two recording modalities through student t-test (p-value ∗ = 0.01, p-value ∗∗ = 0.001, n = 7 recordings, 7 slices for i-MW, and n = 10 recordings, 5 slices for s-MW). H Average jitter percentages were compared among three devices during the more stabilized period and after first 15 min of the recording. The highest value was obtained from i-MW (Student t-test, p-value ∗ <0.04, p-value ∗∗ = 0.001, n = 8 recordings, 5 slices from MEA device, n = 7 recordings, 7 slices from i-MW setting, and n = 12 recordings, 5 slices from s-MW setup). I Average percentage of jitters are calculated within a 1 h time window after 15 min, 1, 6, 12, and 18 h of media exchange. Student t-test was applied to the jitter values of different time points post media exchange. Average jitter percentage is significantly higher during the 1 h time window after 15 min post media changing compared to the other time points (p-value ∗ <0.05, n = 5 slices). J The table summarizes the external disturbances that are present for all three recording platforms. K Duration and interval of seizure-like activities measured from the recordings and normalized fano factors (ratio of variance over mean) are compared between i-MW and s-MW platforms. Fano factors of seizure durations are significantly higher from i-MW recordings (student t-test, p-value < 0.05).