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EC50 values measured for cytotoxicity towards cultured INS-1 cells measured for wild type human amylin and the three consensus sequences. All data are presented as mean ± standard error of mean (SEM).
| EC50 (μM) ± SEM | |||
|---|---|---|---|
| Assay | Alamar Blue | CellTiter-Glo 2.0 | CellTox Green |
| Human amylin | 24.0 ± 0.6 | 22.8 ± 0.7 | 14.7 ± 1.5 |
| Primate consensus | 48.9 ± 1.4 | 46.8 ± 1.0 | 28.8 ± 1.6 |
| Mammalian consensus | 51.8 ± 1.4 | 51.3 ± 1.2 | 36.7 ± 1.2 |
| Vertebrate consensus | 88.3 ± 3.7 | 93.0 ± 7.5 | 54.5 ± 2.8 |
