Table A1.
Primers and probes used in tick‐borne pathogen screening and sequencing
| Oligo name | Primer/probe target | Sequence 5′→3′ | Reference |
|---|---|---|---|
| qPCR: | |||
| Bb23Sf | B. burgdorferi 23S RNA | CGAGTCTTAAAAGGGCGATTTAGT | Courtney et al., 2004 |
| Bb23Sr | B. burgdorferi 23S RNA | GCTTCAGCCTGGCCATAAATAG | |
| Bb23Sp | B. burgdorferi 23S RNA | [FAM]‐AGATGTGGTAGACCCGAAGCCGAGTG‐[BHQ1] | |
| Baf‐RecA‐F | B. afzelii recA | AGTCAGCCTGATACCGGAGA | Klemola et al., 2019 |
| Baf‐RecA‐R | B. afzelii recA | ATTTTGGGGTCAAAGCTGCC | |
| Baf‐RecA‐P | B. afzelii recA | [FAM]‐TGCCGAACATTTAATTAGAAG‐[BHQ1] | Tveten, 2013 |
| Bga‐RecA‐F | B. garinii recA | ATGCAAAAGCTTTGGGGGTT | Klemola et al., 2019 |
| Bga‐RecA‐R | B. garinii recA | AGGGGTTAAAGCTGCTACAGA | |
| Bga‐RecA‐P | B. garinii recA | [HEX]‐TTGCCGAACATTTAATCAGAA‐[BHQ1] | Tveten, 2013 |
| Bbss‐RecA‐F | B. burgdorferi s.s. recA | CCTGATACCGGAGAGCAAGC | Klemola et al., 2019 |
| Bbss‐RecA‐R | B. burgdorferi s.s. recA | GGGGTTAAAGCCGCTACAGA | |
| Bbss‐RecA‐P | B. burgdorferi s.s. recA | [HEX]‐TTGCTGAGCATTTAATCAGAA‐[BHQ1] | Tveten, 2013 |
| Bva‐RecA‐F | B. valaisiana recA | TGGTCCTGAGTCGTCTGGTA | Klemola et al., 2019 |
| Bva‐RecA‐R | B. valaisiana recA | CTTGCTCTCCGGTGTCAGG | |
| Bva‐RecA‐P | B. valaisiana recA | [Cy5]‐AGGTTCAAAAAGAAGGTGGTAT‐[BHQ2] | Tveten, 2013 |
| Bmi‐F | B. miyamotoi glpQ | CACGACCCAGAAATTGACACA | Vayssier‐Taussat et al., 2013 |
| Bmi‐R | B. miyamotoi glpQ | GTGTGAAGTCAGTGGCGTAAT | |
| Bmi‐P | B. miyamotoi glpQ | [FAM]‐TCGTCCGTTTTCTCTAGCTCGATTGGG‐[BHQ1] | |
| Bart‐ssRA‐F | Bartonella ssRa | GCTATGGTAATAAATGGACAATGAAATAA | Diaz et al., 2012 |
| Bart‐ssRA‐R | Bartonella ssRa | GCTTCTGTTGCCAGGTG | |
| Bart‐ssRA‐P | Bartonella ssRa | [FAM]‐ACCCCGCTTAAACCTGCGACG‐[BHQ1] | |
| Rspp‐F | Rickettsia gltA | GAGAGAAAATTATATCCAAATGTTGAT | Labruna et al., 2004 |
| Rspp‐R | Rickettsia gltA | AGGGTCTTCGTGCATTTCTT | |
| Rspp‐P | Rickettsia gltA | [CY5]‐CATTGTGCCATCCAGCCTACGGT‐[BHQ3] | |
| CNeGroEL‐F | Ca. N. mikurensis groEL | CCTTGAAAATATAGCAAGATCAGGTAG | Jahfari et al., 2012 |
| CNeGroEL‐R | Ca. N. mikurensis groEL | CCACCACGTAACTTATTTAGCACTAAAG | |
| CNeGroEL‐P | Ca. N. mikurensis groEL | [FAM]‐CCTCTACTAATTATTGCWGAAGATGTAGAAGGTGAAGC‐[BHQ1] | |
| ApMSP2F | A. phagocytophilum Msp2 | ATGGAAGGTAGTGTTGGTTATGGTATT | Courtney et al., 2004 |
| ApMSP2R | A. phagocytophilum Msp2 | TTGGTCTTGAAGCGCTCGTA | |
| ApMSP2P | A. phagocytophilum Msp2 | [CY5]‐TGGTGCCAGGGTTGAGCTTGAGATTG‐[BBQ650] | |
| Bab18S‐F | Babesia 18S rRNA | CAGCTTGACGGTAGGGTATTGG | Radzijevskaja et al., 2008 |
| Bab18S‐R | Babesia 18S rRNA | TCGAACCCTAATTCCCCGTTA | |
| Bab18S‐P | Babesia 18S rRNA | [HEX]‐CGAGGCAGCAACGG‐[BHQ1] | |
| TBE1‐F | TBEV non‐struct. prot. 5 | GGGCGGTTCTTGTTCTCC | Schwaiger & Cassinotti, 2003 |
| TBE1‐R | TBEV non‐struct. prot. 5 | ACACATCACCTCCTTGTCAGACT | |
| TBE‐Probe‐WT | TBEV non‐struct. prot. 5 | [HEX]‐TGAGCCACCATCACCCAGACACA‐[BHQ2] | |
| FTu23‐F | F. tularensis 23Kda | TGAGATGATAACAAGACAACAGGTAAC | Skottman et al., 2007) |
| FTu23‐R | F. tularensis 23Kda | GGATGAGATCCTATACATGCAGTAGGA | |
| FTu23‐P2 | F. tularensis 23Kda | [HEX]‐CCATTCATGTGAGAACTG‐[BHQ1] | |
| PCR: | |||
| CS877f | Rickettsia gltA | GGGGACCTGCTCACGGCGG | Mediannikov et al., 2004 |
| CS1258r | Rickettsia gltA | ATTGCAAAAAGTACAGTGAACA | |
| Bab‐F | Babesia 18S rRNA | GACACAGGGAGGTAGTGACAAG | Georges et al., 2001 |
| Bab‐R | Babesia 18S rRNA | CTAAGAATTTCACCTCTGACAGT | |
| BbFLA‐F | B. burgdorferi flagellin | AGAGCAACTTACAGACGAAATTAAT | Skotarczak et al., 2002 |
| BbFLA‐R | B. burgdorferi flagellin | CAAGTCTATTTTGGAAAGCACCTAA | |