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. 2020 Oct 12;72(4):1394–1411. doi: 10.1002/hep.31147

Figure 2.

Figure 2

Increased systemic inflammation and hepatic neutrophil infiltration in IRG1−/− mice after I/R. (A) ELISA for plasma IL‐6 levels of WT (C57BL/6NJ) and IRG1−/− mice after sham surgery or 1‐hour ischemia and 6‐hour reperfusion; n = 3 in each sham group, n = 6 in each I/R group. (B‐D) RT‐PCR for mRNA expression of IL‐6, MCP‐1, and Ly6G in livers from WT and IRG1−/− mice after sham surgery or 1‐hour ischemia and 6‐hour reperfusion; n = 3 for sham group, n = 5 for I/R group. (E) MPO levels in livers of WT and IRG1−/− mice after sham surgery or 1‐hour ischemia and 6‐hour reperfusion; n = 3 for sham group, n = 5 for I/R group. (F) Immunofluorescence images of livers from WT and IRG1−/− mice after 1‐hour ischemia and 6‐hour reperfusion; liver tissues were stained with Ly6G (white), Hoecsht nuclear stain (blue), and β‐actin (green); images were taken using confocal microscopy (scale bar, 50 µm). (G) Number of infiltrating neutrophils was determined by Ly6G (no.)/total nuclei (no.) and is represented in bar graph; n = 5 for each group. Images are representative of data from multiple mice per experimental group. Data are presented as means ± SD. *P < 0.05. Abbreviation: DAPI, 4´,6‐diamidino‐2‐phenylindole.