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. 2020 Oct 25;107(10):1423–1432. doi: 10.1002/ajb2.1550

Figure 5.

Figure 5

(A) Alignment of a chromosome 7 physical map for C. sativa (Grassa et al., 2018) with the corresponding linkage group from the segregating F2 population of Weiblen et al. (2015). A single QTL at 38.9 cM, accounting for >90% of variance in the THC:CBD ratio, maps to a region between 20.6 Mb and 31.5 Mb on chromosome 7. Genes for THCAS and CBDAS are located within this region at 26 Mb and 31 Mb, respectively. (B) Model in which THC‐type and CBD‐type plants are reciprocally homozygous for alternately functional (f) and nonfunctional (x) alleles of THCAS and CBDAS. Intermediate‐type plants are heterozygous at both loci. (C) Punnett square for selfing the F1 intermediate of a hybrid cross. According to the close linkage of the loci, we predict that recombinants are not likely to be observed among the F2 descendants of hybrid crosses.