Fig. 1.

Defence against amoebal predation by a subset of V. cholerae strains.

A,D. Amoebal predation was scored using D. discoideum grazing assays in which formed plaques on bacterial lawns were enumerated. Plaque numbers are indicated relative to those formed on a lawn of K. pneumoniae, which served as a positive control. Bar plots represent the average of at least three independent biological replicates (±SD). Statistical significance is indicated (n.s., not significant; ****P < 0.0001).

B,C. Haemolytic activity was tested on blood agar plates. Pandemic V. cholerae strain A1552 as well as all environmental isolates (B) or a representative subset together with their respective hlyA‐minus derivatives (C) were assessed for haemolysis.