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. 2020 Oct 30;72(5):1638–1653. doi: 10.1002/hep.31198

FIG. 1.

FIG. 1

Hepatic ChREBP knockdown reduces downstream enzymes of glycolysis and increases hepatic G6P and glycogen storage in L‐G6pc −/− mice. (A) Box‐and‐whisker plots presenting relative hepatic mRNA levels of Chrebpα and Chrebpβ and relative protein abundance of G6PC and ChREBP in L‐G6pc +/+ and L‐G6pc −/− mice treated with either shChREBP or shSCR (n = 7‐9). (B) Heat maps presenting z score–normalized mRNA expression levels of hepatic glycolysis and PPP enzymes in L‐G6pc +/+ and L‐G6pc −/− mice treated with shChREBP or shSCR (n = 8‐9). (C) Heat maps presenting z score–normalized hepatic activities of glycolytic enzymes in L‐G6pc +/+ and L‐G6pc −/− mice treated with shChREBP or shSCR (n = 6). (D) Heat maps presenting z score–normalized hepatic levels of glycolytic and PPP intermediates in L‐G6pc +/+ and L‐G6pc −/− mice treated with shChREBP or shSCR (n = 7‐9). (E) Box‐and‐whisker plots presenting relative and absolute hepatic glycogen content in L‐G6pc +/+ and L‐G6pc −/− mice treated with shChREBP or shSCR (n = 7‐9). *P < 0.05, **P < 0.01, ***P < 0.001 indicates significance compared with shSCR. ^P < 0.05, ^^^P < 0.001 indicates significance compared with L‐G6pc +/+ mice. Supporting Table S2A‐C contains raw values and statistics for data presented in heat maps. Abbreviations: Aldoa, aldolase, fructose‐bisphosphate A gene; Aldob, aldolase, fructose‐bisphosphate B gene; ChREBP, carbohydrate response element binding protein; Eno1, enolase 1 gene; G6P, glucose‐6‐phosphate; G6pdx, G6P dehydrogenase X gene; Gapdh, glyceraldehyde‐3‐phosphate dehydrogenase gene; Gck, glucokinase gene; Gckr, glucokinase regulator gene; Gpi1, G6P isomerase 1 gene; L‐G6pc −/−, liver‐specific glucose‐6‐phosphatase catalytic subunit knockout; L‐G6pc +/+, liver‐specific glucose‐6‐phosphatase catalytic subunit–sufficient; Pfkl, phosphofructokinase, liver type gene; Pgd, phosphogluconate dehydrogenase gene; Pgk1, phosphoglycerate kinase 1 gene; Pgls, 6‐phosphogluconolactonase gene; Pgm1, phosphoglucomutase 1 gene; Pgm2, phosphoglucomutase 2 gene; Pgm3, phosphoglucomutase 3 gene; Pklr, pyruvate kinase L/R gene; PPP, pentose phosphate pathway; Rpe, ribulose‐5‐phosphate‐3‐epimerase gene; Rpia, ribose 5‐phosphate isomerase A gene; shChREBP, short hairpin ChREBP; shRNA, short hairpin RNA; shSCR, scrambled shRNA; Taldo1, transaldolase 1 gene; Tkt, transketolase gene; Tpi1, triosephosphate isomerase 1 gene.