Skip to main content
. 2020 Sep 24;252(3):e5517. doi: 10.1002/path.5517

Figure 4.

Figure 4

Adam10 knockdown causes abnormal ex vivo cultured embryonic kidney development. (A) Schematic diagram of the Adam10 morpholino (MO) design. (B, C) Adam10‐MO knockdown efficiency was confirmed by PCR and western blotting using IMCD cells. (D) Whole‐mount fluorescence microscope images of ex vivo cultured E12.5 Six2‐eGFP/Rosa26mT mice kidneys treated with Adam10‐MO (right) or not (left). Scale bars: 200 μm. (E) Whole‐mount fluorescence microscope images of ex vivo cultured E12.5 normal mice kidneys treated with Adam10‐MO (right) or not (left). The expression of SIX2, LTL, N1ICD, N2ICD, and HES1 was detected. Scale bars of other columns: 100 μm.