Table 2.
Primer sequences and TP‐ARMS‐PCR conditions for rs4774388 and rs11639084
| SNP | Primer | Primer Sequence | Amplicon Size (bp) | Ta (°C) |
|---|---|---|---|---|
| rs4774388 | FO | CTGTGAGACCCTTTGACAACAGTACGTG | 412 | |
| RO | TGAGGAAGAGTCCTTAGGAAGGGATGTC | 65 | ||
| FI | CGGCATGATATTCATCGTCAAATCTGTTGC | 197 (C allele) | ||
| RI | GCTGAGATGGAGACATCACAGAATGTCA | 273 (T allele) | ||
| rs11639084 | FO | GTATTTGCATTTGTCATCCTTATCAACC | 321 | |
| RO | AACTGTGGGCAAGTTATTTAACCTCTCT | 57 | ||
| FI | GTTGCCAGCTAATGTTTATTGCATAATC | 162 (C allele) | ||
| RI | TGGAGGCTTTAGTCTCTGGAACATATTA | 215 (T allele) |
The nucleotide specificity is indicated in parentheses.
Abbreviations: F, forward; I, inner; O, outer; R, reverse; SNP, Single‐nucleotide polymorphism; Ta, annealing temperature.