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. 2020 Nov 25;36(12):998–1009. doi: 10.1089/aid.2020.0163

FIG. 2.

FIG. 2.

Generation of fluorescent targets. (A) Scheme of the SIVmac239 genome with the IRES-FP cassette inserted downstream of the nef gene. (B) IncuCyte image and flow cytometry analysis of EGFP or mRuby3 expression in purified CD4 T cells infected with the SIV-EGFP (left) or SIV-mRuby3 (right) virus. EGFP, enhanced green fluorescent protein; FP, fluorescent protein; IRES, internal ribosome entry site.