Table 2.
Composition of qPCR reaction.
| Component | Volume (μL) |
|---|---|
| cDNA | 3.0 |
| Forward primer (10 μm) | 1.0 |
| Reverse primer (10 μm) | 1.0 |
| 2x RealStar Green Power Mixture | 5 |
| Mix | 10 |
| TaqE | 0.2 |
| RNase-free H2O | 4.8 |
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