Fig. 7.

Insulin signaling and dose–response relationships. (A) Similar activities of WT insulin (HI; blue diamond) and Orn^B29-insulin (Orn^B29-HI; red square). Ligand concentrations are shown in horizontal axis. (B) The dose–response relationship of Tyr^B24, Orn^B29-insulin (blue circle) is shifted to the right relative to Orn^B29 parent (red square). Superimposed as a green line is the activity of des[B30]-porcupine insulin. (C) Dose–response relationships of des[B30]-porcupine insulin (green triangle) relative to des[B30]-Orn^B29 human insulin (red square). Ligand doses: 0.05, 0.15, 0.5, 5, 10, 30, 100, 300, and 900 nM; assays were performed in triplicate.