Skip to main content
. 2020 Nov 9;117(47):29914–29924. doi: 10.1073/pnas.2009224117

Fig. 1.

Fig. 1.

Dstac and Dmca1D are expressed by proctolin+ motor boutons. (A) Anti-proctolin and anti- horseradish peroxidase (anti-HRP) labeling of motor nerves in larvae selectively expressing membranous GFP in proctolin+ motor neurons (Proct:GAL4 > UAS:mCD8GFP) showed expression of mCD8GFP and proctolin at type Ib and type Is branches. The anti-HRP labels an unknown protein that is present on the plasma membrane of motor nerves and boutons. Shown are the type Is branch of the RP2 (arrow) and the type Ib branch of the MN4-Ib motor neuron (asterisk) on muscle 4 (45). Merge is Proct:GAL4 > UAS:mCD8GFP and anti-Proctolin. The images are a single focal plane. (Scale bar: 3 μm.) (B) Anti-Dstac and anti-HRP labels type 1b (asterisk) and 1s (arrow) boutons. Left Inset shows higher magnification view of type 1b boutons. Right Inset shows higher magnification view of type 1s boutons. Shown are type 1s boutons of RP2 and type 1b boutons of MN4-1b on muscle 4. (C) Anti-Dmca1D and anti-HRP labels type 1b (asterisk) and 1s (arrow) boutons. Left Inset shows higher magnification view of type 1b boutons. Right Inset shows higher magnification view of type 1s boutons. Shown are type 1s boutons of RP2 and type 1b boutons of MN4-1b on muscle 4. Some of the labeling by both anti-Dstac and anti-Dmca1D beyond the motor nerves may represent muscle labeling since body wall muscles also express Dstac and Dmca1D (38, 46). The images show a single focal plane. (Scale bar: 3 μm.) See also SI Appendix, Fig. S1.