Fig. 1.

Cryo-EM reconstructions of the mature and assembly intermediate L. tarentolae mitoribosome. (A) Composite cryo-EM map of the full and mature mitoribosome of L. tarentolae. SSU is depicted in orange shades, with proteins in orange and rRNA in yellow, and LSU is depicted in blue, with proteins in dark blue and rRNA in aquamarine. (B) Relative sizes of the rRNA (colored segmented densities) compared with the r-proteins (thick black contours). (C–F) Cryo-EM reconstructions of the mature LSU viewed from the intersubunit (C) and solvent (D) sides, compared with the LSU assembly intermediate subunit (E and F). Densities corresponding to assembly factors are shown in purple; gray represents unidentified factors.