Fig. 3.

Identification of the residues of TMH 6 and TMH 12 that govern the import of substrates by the 14A mutant. (A) Schematic showing residues mutated in different P-gp variants in TMH 6 (red ovals) and TMH 12 (magenta-filled ovals). (B) Heatmap showing the accumulation of Rh123, DHR123, Rhod-2 AM, and Flutax-1 by 14A and other mutants. The heat map shows the relative levels of accumulation of each substrate, with the darkest being the highest level of uptake of a given substrate (white panels indicate no accumulation).