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. 2020 Sep 12;10(3):297–307. doi: 10.4314/ovj.v10i3.8

Table 1. Oligonucleotide primers’ sequences and PCR conditions.

Target agent Length of amplified product Primer sequence (5’-3’) Gene Reference
S. aureus 102 bp GGTTATCAATGTGCGGGTGG sea Mehrotra et al., 2000x
CGGCACTTTTTTCTCTTCGG
278 bp CCAATAATAGGAGAAAATAAAAG sed
ATTGGTATTTTTTTTCGTTC
209 bp AGGTTTTTTCACAGGTCATCC see
CTTTTTTTTCTTCGGTCAATC
164 bp GTATGGTGGTGTAACTGAGC seb
CCAAATAGTGACGAGTTAGG
451 bp AGATGAAGTAGTTGATGTGTATGG sec
CACACTTTTAGAATCAACCG
Primary denaturation: 94°C/5 minutes; secondary denaturation: 94°C/30 seconds; annealing: 57°C/40 seconds; extension: 72°C/45 seconds; No. of cycles: 35; final extension: 72°C/10 minutes.
1250 bp AC GGAGTTACAAAGGACGAC 23S rRNA Straub et al., 1999
AGCTCAGCCTTAACGAGTAC
Primary denaturation: 94°C/5 minutes; secondary denaturation: 94°C/30 seconds; annealing: 55°C/40 seconds; extension: 72°C/1.2 minutes.; No. of cycles: 35; final extension: 72°C/12 minutes.
E. coli 266 bp ATGTTATTTTCTGCCGCTCTG Iss Yaguchi et al., 2007
CTATTGTGAGCAATATACCC
Primary denaturation: 94°C/5 minutes; secondary denaturation: 94°C/30 seconds; annealing: 54°C/30 seconds; extension: 72°C/30 seconds; No. of cycles: 35; final extension: 72°C/7 minutes.
614 bp ACACTGGATGATCTCAGTGG Stx1 Dipineto et al., 2006
CTGAATCCCCCTCCATTATG
Primary denaturation: 94°C/5 minutes; secondary denaturation: 94°C/30 seconds; annealing: 58°C/40 seconds; extension: 72°C/45 seconds; No. of cycles: 35; final extension: 72°C/10 minutes.
508 bp TGCAGAACGGATAAGCCGTGG fimH Ghanbarpour and Salehi, 2010
GCAGTCACCTGCCCTCCGGTA
Primary denaturation: 94°C/5 minutes; secondary denaturation: 94°C/30 seconds; annealing: 50°C/40 seconds; extension: 72°C/45 seconds; No. of cycles: 35; final extension: 72°C/10 minutes.
620 bp GGT GGT GCA CTG GAG TGG Tsh Delicato et al., 2003
AGT CCA GCG TGA TAG TGG
Primary denaturation: 94°C/5 minutes; secondary denaturation: 94°C/30 seconds; annealing: 54°C/40 seconds; extension: 72°C/45 seconds; No. of cycles: 35; final extension: 72°C/10 minutes.
720 bp CGATTCTGGAAATGGCAAAAG phoA Hu et al., 2011
CGTGATCAGCGGTGACTATGAC
Primary denaturation: 94°C/5 minutes; secondary denaturation: 94°C/30 seconds; annealing: 55°C/40 seconds; extension: 72°C/45 seconds; No. of cycles: 35; final extension: 72°C/10 minutes.