Table 3. Investigated bacteria and their isolation and identification procedures.
| Investigated bacteria | Media | Incubation |
|---|---|---|
| APC | Plate count agar (PCA, CM: 325, Oxoid, UK). | 30°C for 48 hours |
| Enterococci spp. | ESD: prepared in the lab. | 37°C for 24 hours |
| Bacillus spp. | (MYP Agar Base, M035, Park Scientific limited, UK) supplemented with egg yolk emulsion (SR: 047C, Oxoid, UK) and polymyxin B (SR 099, Oxoid, UK). | 37°C for 24 hours |
| EHEC O157:H7 | MacConkey agar (SMAC, CM: 0813, Oxoid, UK) supplemented with cefotaxime tellurite (SR: 172, Oxoid, UK). | 37°C for 24 hours |
| Staphylococci spp. | BP Agar (CM: 275, Oxoid, UK) supplemented with egg yolk tellurite emulsion (SR: 54, Oxoid, UK). | 37°C for 24-48 hours |
| Vibrio spp. | Vibrio spp. selective TCBS plates (TCBS: # 611010, Liofilchem, Italy). | 37°C for 48 hours |
| Cronobacter spp. | HiChrome Ent. Sakazakii modified agar, (M1641, HiMedia, India). | 44°C for 24 hours |
| Salmonella spp. | Bismuth sulfite (BS) agar (CM: 0201, Oxoid, UK) and xylose lysine desoxycholate (XLD) agar (CM: 469, Oxoid, UK). | 35°C for 24 hours |
APC = aerobic plate count.