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. 2020 Nov 9;117(47):29823–29831. doi: 10.1073/pnas.2019604117

Fig. 4.

Fig. 4.

Infiltrated immune cells contribute to the tumor-suppressive effect of p50. (A and B) The suppressive effect mediated by p50 is partially abrogated in tumors grown in NSG mice lacking active macrophages and NK cells. Growth rates (i) and weights (ii) of tumors derived from U87-MG cells expressing V0 or FLAG-p50 in SCID and nude (A) or in NSG and nude (B) mice. (iii) The corresponding tumors 4 wk after inoculation in either SCID (A, iii), NSG (B, iii), or nude (A, iii and B, iii) mice. (iv) Immunohistochemical staining for CD45 of U87-MG cells overexpressing V0 or FLAG-p50 in nude and SCID (A, iv), or in nude and NSG (B, iv) mice. (C) Growth rates (i) and weights (ii) of tumors derived from U87-MG cells expressing V0 or FLAG-p50 in NSG mice in the presence (reconstituted) or absence of mouse NK cells. (iii) Immunohistochemical staining for NKG2D in tumors derived from U87-MG cells expressing V0 or FLAG-p50 in the presence (reconstituted) or absence of mouse NK cells. (D) Growth rates (i) and weights (ii) of tumors derived from U87-MG cells expressing V0 or FLAG-p50 in NSG mice in the presence (reconstituted) or absence of human NK cells. (E) Growth rates (i) and weights (ii) of tumors derived from U87-MG cells expressing V0 or FLAG-p50 in NSG mice in the presence (reconstituted) or absence of mouse monocytes or monocytes and NK cells. * in the relevant panels represents P value < 0.05. NS represents nonsignificant.