Fig. 7.

CarD_Rsp levels at different phases of growth. (A) Western blots were performed from R. sphaeroides aerobic cultures grown at 30 °C, diluted from stationary phase into fresh minimal medium at time 0, and sampled at the indicated times. Separate experiments were conducted for each growth phase (outgrowth, log, and stationary; detailed in SI Appendix, Expanded Materials and Methods). For each sample, the OD₆₀₀ was measured, CarD levels were determined by Western blotting with anti-CarD_Rsp antibody, and RNAP_Rsp levels were determined with an anti–α-subunit antibody. Purified CarD standards were analyzed in parallel for quantitation of CarD levels. (B) Relative CarD levels and OD₆₀₀ values for cultures sampled during outgrowth, log, and stationary phase. (C) Relative RNAP_Rsp α-subunit levels in the same samples analyzed for CarD levels. Further details are provided in SI Appendix, Expanded Materials and Methods. Values for CarD_Rsp, RNAP_Rsp α-subunit, and OD₆₀₀ were plotted on the same scale by normalizing to the CarD level at an overlapping OD_600. Error ranges were determined from two experiments for each.