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Enzalutamide-resistant LNCaP (MR49F) cellular antiproliferation. Enzalutamide (4)-resistant (Enz-R) LNCaP (MR49F) cells were plated in 1% charcoal-stripped, serum-containing medium and treated with 0.1 nM R1881 and a titration of antagonist as indicated in the figure. Cells were retreated 3 d after the first treatment and the number of viable cells was measured by the CellTiter-Glo assay (Promega, Madison, WI). N = 3.
