Table 3.
List of Reagents and Resources
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| TDP-43-Oligo01 | In House | In House |
| TDP-43-Oligo02 | In House | In House |
| A11 | In House | In House |
| OC | In House | In House |
| TDP-43 | Abcam | ab109535 |
| pTDP-43 | CosmoBio | TIP-PTD-M01 |
| TDP-43 – Alexa Fluor 568 | Abcam | ab208544 |
| Tau13 | BioLegend | MMS-520R |
| Tau5 | BioLegend | 806402 |
| TOMA-2 | In House | In House |
| 6E10 | BioLegend | 803002 |
| GAPDH | Abcam | ab9485 |
| LaminB1 | Abcam | ab133741 |
| Histone3 | Abcam | ab201456 |
| Alexa Fluor Anti-Mouse 488 | Invitrogen | A11029 |
| Alexa Fluor Anti-Rabbit 488 | Invitrogen | A11034 |
| Alexa Fluor Anti-Rabbit 568 | Invitrogen | A11036 |
| Alexa Fluor Anti-Mouse 568 | Invitrogen | A11031 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMEM | Gibco | 11965–092 |
| Antibiotics | Gibco | 15240–062 |
| FBS | Gibco | 16000–044 |
| Paraformaldehyde, 4% | Electron Microscopy Sciences | 15714-S |
| Triton-X 100 | Sigma | T8787 |
| Protease Inhibitor | Thermo Scientific | A32953 |
| Tween | Fisher Scientific | BP337–500 |
| FBS-depleted DMEM | Gibco | LS11965118 |
| PBS | Corning | 46–013-CM |
| Gibco™ Trypsin-EDTA, 0.25% Phenol red | Fisher Scientific | LS25200114 |
| Goat serum | Sigma | G90223–10mL |
| Accutase | Sigma-Aldrich | A6964–100ML |
| B27 Supplemental | Gibco | A3582801 |
| Amphotericin B | Gibco | 15290018 |
| Neurobasal™ medium | Gibco | 12348017 |
| L-glutamine | Hyclone | SH30034.01 |
| Critical Commercial Assays | ||
| Qproteome Cell Compartment Kit | Qiagen | #37502 |
| Alexa Fluor™ 568 NHS Ester | Invitrogen | A20003 |
| Lipofectamine™ RNAiMAX Transfection Reagent | Thermo Scientific | 13778030 |
| Pierce™ Co-Immunoprecipitation Kit | Thermo Scientific | #26149 |
| Micro BCA Kit | Thermo Scientific | #23235 |
| Experimental Models: Cell Lines | ||
| HEK-293 | Dr. Laura Blair | |
| WT-6 tau iHEK | Dr. Laura Blair | |
| P301L tau iHEK | Dr. Laura Blair | |
| Software and Algorithms | ||
| GraphPad Prism 6 | Graphpad.com | |
| ImageJ FIJI | ImageJ | NIH |
| Analyzer BZ-X | Keyence Microscope | Keyence Company |
| Other | ||
| 10kDa Amicon Ultra-0.5 Centrifugal Filter Units | Millipore | UFC501096 |
| ProLong™ Gold Antifade Mountant with DAPI | Invitrogen | P36962 |