Fig. 1.

MitoTrack GUI to track transcriptional activation and nuclei lineage. (a) Main window composed of four distinct panels, among which, two are shown here, with raw nuclei channel (upper left) and detected nuclei (upper right). MitoTrack typical tools and edit boxes are shown on the left. (b) ‘Modifier Mitosis Tool’ showing all its commands and parameters. Three typical images of nuclei at a time t, t + 1 and their overlap are shown to illustrate the basis of the algorithm for automatic nuclei tracking during mitosis. (c) Interface of the ‘Modify Segmentation Tool’ with its main commands visible. Exemplary results of manual corrections of nuclei segmentation