RES blocks the formation of autolysosomes by suppressing actin remodeling. (A) RES inhibits formation of autolysosomes. A431 cells treated as in Fig. 1A followed by subcellular detection of Lamp-2 and LC3 by immunofluorescence microscopy using anti-Lamp-2 and -LC3 antibodies. Green, Lamp-2; red, LC3; yellow, Lamp-2 ⁄ LC3; blue, DAPI. Magnification 200×. (B) Unpolarized filopodia are increased in A431 cells treated with RES as in Fig. 1A (b, white arrows). F-actin was stained using rhodamine-phalloidin. DAPI, blue.